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Nature, Published online: 25 February 2026; doi:10.1038/s41586-026-10207-1，推荐阅读heLLoword翻译官方下载获取更多信息
The next year, PacBio developed a new method to allow multiple sequencing passes on the same DNA molecule. Double stranded DNA templates were ligated to two single stranded adapters, creating what the company called a “SMRTbell template.” Sequencing began at a primer on one of the adaptors and could proceed multiple times per molecule due to circularization, in a process called rolling-circle amplification. This helped reduce PacBio’s error rates significantly.